Protocol for Cohesionless Sample Preparation for Physical Experimentation

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Sample preparation for STED microscopy.

Since the discovery of the diffraction barrier in the late nineteenth century, it has been commonly accepted that with far-field optical microscopy it is not possible to resolve structural details considerably finer than half the wavelength of light. The emergence of STED microscopy showed that, at least for fluorescence imaging, these limits can be overcome. Since STED microscopy is a far-fiel...

Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis

A major limitation for better understanding the role of the human gut virome in health and disease is the lack of validated methods that allow high throughput virome analysis. To overcome this, we evaluated the quantitative effect of homogenisation, centrifugation, filtration, chloroform treatment and random amplification on a mock-virome (containing nine highly diverse viruses) and a bacterial...

A Simple Sample Preparation with HPLC-UV Method for Estimation of Clomipramine from Plasma

Clomipramine is a tricyclic antidepressant. Different methods for determination of clomipramine hydrochloride in plasma have been described. Most of these procedures favor the use of acidic back-extraction in extraction procedure and HPLC as the analytical technique. In this study, the clomipramine extraction procedure was modified and a direct injection to the column was performed to shorten t...

Vibrating Sample Holder for Xrd Analysis with Minimal Sample Preparation

A new approach to sample handling for powder X-ray diffraction analysis is presented that allows characterization of poorly prepared samples. The technique relies on vibrations generated in a sample holder to create movement of the powdered sample in the absence of macroscopic movement of the sample holder itself. The major benefits over conventional sample handling strategies are: 1) the abili...